Coding

Part:BBa_K137006:Design

Designed by: Victoria Hsiao   Group: iGEM08_Caltech   (2008-06-20)


pabB (from pABA synthesis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The start codon of pabB in L.lactis is TTG, which cannot be used in E.coli. So we designed the primer such that the first T was omitted and the A from the xba1 primer make an ATG start codon.


Source

L.lactis genome

References